Zeiss LSM 880 Operating Manual page 513

LSM 880 Center Screen Area / Image Containers - Display and Image Analysis
Thresholds tab (Fig. 702):
The threshold for the image analysis can be set
manually using the slider or editing text box next
to Donor, Acceptor, FRET or All, where All
moves all sliders to the same value. Alternatively,
the threshold can be set by defining and enabling
a background region in the FRET tab. The values
for the thresholds are either displayed as grey value
levels (Raw data) or Normalized to the value 1.
Settings tab (Fig. 698 and Fig. 703):
This tab provides access to the definition of the
parameters used for the image analysis.
The General (Fig. 698) tab applies to both
methods, Acceptor Photobleaching and Sensitized
Emission.
If checked the listed options will be taken into
account for the analysis of FRET.
The Sensitized Emission (Fig. 703) tab sets
additional Truncation parameters for each method
to analyze the Sensitized Emission experiment such
as the choice for No truncation, Remove
maximum or the truncation of values above 1
(<=1), 2 (<=2) or 3 (<=3).
For the Fc analysis method, the option Don't
show negative values in the Fc image can be
selected using the check box. With the FRET-N
normalization slider and text box a multiplication
factor for the calculated FRET-N values can be set.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Fig. 702
Fig. 703
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FRET View Options Control Block,
Thresholds tab
FRET View Options Control Block,
Settings tab, Sensitized Emission
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