Zeiss LSM 880 Operating Manual page 376

CHAPTER 1 - SYSTEM OPERATION
ZEISS Left Tool Area and Hardware Control Tools LSM 880
For Mean Fusion and Maximum Fusion the checkbox Fusion Subset in X with Preview can be
activated (Fig. 523). With these controls it is possible to define which area of the left and the right
illumination image should be used for fusion.
When this function is chosen, the image is displayed in the Preview Tab in the image container
(Fig. 524). In this preview mode the left hand image shows the original dataset and the right hand image
the preview of processing. Using the dimension sliders within the image control area the Z-Position,
Illumination side and View can be chosen.
In Fusion Subset in X with Preview the currently chosen View is displayed in the Current View box.
With the Left slider or input box the area of the left illumination side is chosen. This is marked at the
slider itself with a red line, the input box shows the pixel value along the y axis. Furthermore the cut off is
shown in the preview image with a red line for easy orientation. It is best to set the illumination slider
of the image control area to 1, showing the left side illumination of the original data.
Repeat the process with the Right slider and input box; here the marking lines are in blue and the
illumination slider in the image control area should be placed to 2, showing the right side
illumination of the original data.
The Preview in the image container will show the position of both sliders Left and Right as red and blue
lines respectively (Fig. 524).
To avoid a visible change in intensity along the two cut off lines, for Mean Fusion with Fusion Subset
in X, an overlap of both illumination sides should remain in the center of the image and the Blending
slider should be used to define the number of pixels used within this overlap to crossfade both
illuminations sides.
The position of the sliders Left and Right as well as the Blending value must be defined for each view
individually.
Fig. 523 Dual Side Fusion – Fusion Subset in X
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