1. Manuals
  2. Brands
  3. Zeiss Manuals
  4. Microscope
  5. LSM 510 META
  6. Operating manual

Zeiss LSM 510 Operating Manual

Laser scanning microscopes
Hide thumbs Also See for LSM 510:
Table of Contents

Advertisement

Quick Links

LSM 510 and LSM 510 META
Laser Scanning Microscopes
Operating Manual
Release 3.2

Advertisement

Table of Contents
loading

Related Manuals for Zeiss LSM 510

Summary of Contents for Zeiss LSM 510

  • Page 1 LSM 510 and LSM 510 META Laser Scanning Microscopes Operating Manual Release 3.2...
  • Page 2 LSM 510 Carl Zeiss LSM 510 META Knowledge of this manual is required for the operation of the instrument. Would you therefore please make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument.
  • Page 3 INTRODUCTION LSM 510 META Carl Zeiss How to make best use of the LSM 510 operating instructions A few symbols in these operating instructions will help you to recognize the nature and purpose of information immediately: The WARNING symbol warns against hazards for the user that might arise when operating the laser.
  • Page 4 Axiovert 200 M SP 000000-1115-092 Axiovert 200 M BP 000000-1115-094 Axiovert 200 M BP/SP 000000-1115-289 Objectives: ............................Confocal Laser Scanning Module LSM 510 Configuration 1 000000-1219-975 Configuration 2 000000-1219-977 Configuration 3 000000-1219-978 Configuration 4 000000-1219-979 Configuration 5 000000-1219-980...
  • Page 5 LSM 510 INTRODUCTION LSM 510 META Carl Zeiss Optional software: Image VisArt option 000000-1207-934 3D for LSM option 000000-1207-919 3D Deconvolution option 000000-1207-920 Physiology option 000000-1207-930 Topography option 000000-1207-933 Multiple Time Series option 000000-1207-926 Macro Reader and Editor option 000000-1207-925...
  • Page 6 INTRODUCTION LSM 510 Carl Zeiss LSM 510 META The LSM 510 in the configuration as checked above was installed and handed to the customer in functional condition ....................Phone: ..........Fax: ..........The customer has been instructed on how to operate and maintain the equipment.
  • Page 7 Here you will find an introduction to Laser Scanning Microscopy, with an explanation of the principles of confocal imaging. The section also outlines the ways to present LSM image series in three dimensions, and introduces you to the performance features of your LSM 510. Quickstart...
  • Page 8 INTRODUCTION LSM 510 Carl Zeiss LSM 510 META VIII B 45-0008 e 10/02...

  • Page 9: Table Of Contents

    LSM 510 NOTES ON DEVICE SAFETY LSM 510 META Contents Carl Zeiss CHAPTER 1 NOTES ON DEVICE SAFETY CONTENTS Page NOTES ON DEVICE SAFETY....................1-3 General...........................1-3 Regulations ..........................1-3 Notes on Setting up the Microscope System................1-4 Notes on Handling the Computer and Data Media ..............1-5 Notes on Care, Maintenance and Service .................1-6...
  • Page 10 NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss Contents LSM 510 META B 45-0008 e 10/02...

  • Page 11: Notes On Device Safety

    The manufacturer will not assume liability for any malfunction or damage caused by any thing other than the intended use of the LSM 510 or individual modules or parts of it, nor by any repair or other service operation performed or attempted by persons other than duly authorized service staff.

  • Page 12: Notes On Setting Up The Microscope System

    • Crate 8: upgrade kit META The LSM 510 must be set up so as to ensure that the minimum clearance between the wall and the rear of the system is no less than 0.5 m. This clearance is needed for adjustment and maintenance operations.

  • Page 13: Notes On Handling The Computer And Data Media

    LSM 510 NOTES ON DEVICE SAFETY LSM 510 META Notes on Handling the Computer and Data Media Carl Zeiss Notes on Handling the Computer and Data Media The computer used as standard in your LSM system is an IBM-compatible high-end pentium computer with WINDOWS NT 4.0 or WINDOWS 2000 operating system.

  • Page 14: Notes On Care, Maintenance And Service

    To complete cleaning, lightly breathe on the glass surface and rub it dry with a clean cloth. Lint or dust is best removed with a clean hairbrush. The air filter mat at the bottom of the LSM 510 Control Unit must be cleaned every six months. Filter mats can be ordered from our Service Department.

  • Page 15: Notes On Handling The Laser Components

    If used properly, the LSM 510 will not pose any laser radiation risks for operating staff. The dangerous laser radiation area is limited to the beam path and to a distance of up to around 10 cm from the specimen.

  • Page 16: Warning And Information Labels

    The warning and information labels attached on the LSM 510 must be observed. Check whether all of the labels shown below are provided on your instrument, and contact Carl Zeiss Germany or one of the service agencies if you should discover that any of the labels should be missing.
  • Page 17 LSM 510 META NOTES ON DEVICE SAFETY LSM 510 Warning and Information Labels Carl Zeiss Fig. 1-2 Warning and information labels on the Axioplan 2 imaging microscope with LSM 510 META scanning module B 45-0008 e 10/02...
  • Page 18 NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss Warning and Information Labels LSM 510 META Fig. 1-3 Warning and information labels on laser components (page 1) 1-10 B 45-0008 e 10/02...
  • Page 19 LSM 510 META NOTES ON DEVICE SAFETY LSM 510 Warning and Information Labels Carl Zeiss x im te r s s u p re .I . p .s te r R I S Fig. 1-3 Warning and information labels on laser components (page 2)
  • Page 20 NOTES ON DEVICE SAFETY LSM 510 Carl Zeiss LSM 510 META 1-12 B 45-0008 e 10/02...
  • Page 21 Laser Module VIS (405, 458, 477, 488, 514, 543, 633 nm)..........2-16 2.11 Laser Module UV (351, 364 nm) ..................2-16 2.12 System Overview LSM 510 META................... 2-17 2.13 System Overview LSM 510 META - NLO ................. 2-19 B 45-0008 e 10/02...
  • Page 22 LSM 510 - SETUP REQUIREMENTS LSM 510 Carl Zeiss Contents LSM 510 META B 45-0008 e 10/02...

  • Page 23: Lsm 510 - Setup Requirements

    LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META Space Requirements Carl Zeiss LSM 510 - SETUP REQUIREMENTS Space Requirements LSM (one microscope, large system table): 300 × 250 cm 2.1.1 Power Plug System rack 1000 2500 Large system table...

  • Page 24: Lsm With Ar Uv Laser

    LSM 510 - SETUP REQUIREMENTS LSM 510 Carl Zeiss Space Requirements LSM 510 META 2.1.2 LSM with Ar UV Laser ☞ We recommend placing the cooling unit of the Ar laser (UV) in a separate room to prevent heat accumulation and vibration. Length of the water hose: 400 cm...
  • Page 25 LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META Space Requirements Carl Zeiss Two microscopes: Power Plugs To Cooling Unit System System Rack I Rack II 1000 3000 Small Large system table system table 1500 3500 Fig. 2-3 The system rack I contains the VIS laser module (Helium-Neon laser 543 nm, 633 nm and Argon ion laser) and the Argon UV laser module.

  • Page 26: Lsm Prepared For Two Photon Lasers (Nlo)

    LSM 510 - SETUP REQUIREMENTS LSM 510 Carl Zeiss Space Requirements LSM 510 META 2.1.3 LSM prepared for Two Photon Lasers (NLO) 2.1.3.1 Coherent "Mira 900" Direct-coupling with Inverted Stand (Upright Stand also possible) Power Plug LM VIS Power Supplies...
  • Page 27 LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META Space Requirements Carl Zeiss 2.1.3.3 Coherent "Mira" Fiber-coupling with Inverted Stand (Upright Stand also possible) Power Plug LM VIS 1400 System Rack 1000 Large NLO 1800 2500 system table Large system table...

  • Page 28: Power Requirements

    Power Requirements ☞ The LSM 510 comes with a mains power supply cord and plug, either CEE red (230 V, 16 A, 3 phases), or CEE yellow (115 V, 32 A, 3 phases), and with the matching mains socket outlet.

  • Page 29: Phase 1 (Lsm)

    LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META Power Requirements Carl Zeiss Power distribution inside the Laser Module VIS: Fig. 2-8 2.2.1 Phase 1 (LSM) feeds the following units: Laser Module HeNe 2× via Power 1 (5-socket adapter)

  • Page 30: Physical Dimensions

    LSM 510 - SETUP REQUIREMENTS LSM 510 Carl Zeiss Physical Dimensions LSM 510 META Physical Dimensions Length (cm) Width (cm) Height (cm) Weight (kg) Large system table Small system table Active anti-vibration table Active anti-vibration table (NLO) For Mai Tai Laser or Chameleon...

  • Page 31: Dimension Of Shipment Crates

    LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META Dimensions ... / Environmental Requirements Carl Zeiss Dimension of Shipment Crates Crate containing Length (cm) Width (cm) Height (cm) Weight (kg) Large system table Small system table Active anti-vibration table...

  • Page 32: Vibrations

    LSM 510 - SETUP REQUIREMENTS LSM 510 Carl Zeiss Vibrations / Laser Specifications LSM 510 META Vibrations Vibrations under operation conditions Shipping shock (LSM 510 box) (with system table) 5 µm pp at 5 Hz 10 µm pp at 10 Hz 10 µm pp at 20 Hz...

  • Page 33: Lasos Lgk 7786 P / Power Supply 7460 A: 543 Nm, 1 Mw, Laser Class 3 B

    LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META Laser Specifications Carl Zeiss 2.7.3 LASOS LGK 7786 P / Power supply 7460 A: 543 nm, 1 mW, Laser Class 3 B Line voltage 115/230 V with factory setting Line frequency 50...60 Hz...

  • Page 34: Microscopes

    Upright Axiotron 2 mot Inverted Axiovert 200 M BP or SP Upright Axioskop 2 FS Upright Axioskop 2 MAT mot All Zeiss ICS objectives and accessories can be accommodated. Z motor DC servomotor, opto-electronically coded Least Z interval: 50 nm...

  • Page 35: Scanning Module

    LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META Scanning Module Carl Zeiss Scanning Module 2 individually driven galvanometric scanners Up to ~5 frames/sec (512 × 512 pixels) Scanning speed Max. 2048 × 2048 pixels (individually adjustable for each axis) Field resolution 10 ×...

  • Page 36: Laser Module Vis (405, 458, 477, 488, 514, 543, 633 Nm)

    LSM 510 - SETUP REQUIREMENTS LSM 510 Carl Zeiss Laser Module VIS / Laser Module UV LSM 510 META 2.10 Laser Module VIS (405, 458, 477, 488, 514, 543, 633 nm) Single-mode polarization preserving fiber Laser beam attenuation for all lasers by VIS-AOTF...

  • Page 37: System Overview Lsm 510 Meta

    LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META System Overview LSM 510 META Carl Zeiss 2.12 System Overview LSM 510 META B 45-0008 e 10/02 2-17...
  • Page 38 LSM 510 - SETUP REQUIREMENTS LSM 510 Carl Zeiss System Overview LSM 510 META LSM 510 META 2-18 B 45-0008 e 10/02...

  • Page 39: System Overview Lsm 510 Meta - Nlo

    LSM 510 LSM 510 - SETUP REQUIREMENTS LSM 510 META System Overview LSM 510 META - NLO Carl Zeiss 2.13 System Overview LSM 510 META - NLO B 45-0008 e 10/02 2-19...
  • Page 40 LSM 510 - SETUP REQUIREMENTS LSM 510 Carl Zeiss System Overview LSM 510 META - NLO LSM 510 META 2-20 B 45-0008 e 10/02...
  • Page 41 Three-Dimensional Presentations of LSM Image Stacks .............3-4 Optical Diagram of the LSM 510 (Schematic)..............3-6 Performance Features of the LSM 510................3-7 3.4.1 Optical and Mechanical Aspects ..................3-7 3.4.2 Microscope Equipment of the LSM 510 System ..............3-8 3.4.3 Computer Hardware and Software ................3-10 B 45-0008 e 10/02...
  • Page 42 INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 Carl Zeiss Contents LSM 510 META B 45-0008 e 10/02...

  • Page 43: Introduction To Laser Scanning Microscopy

    LSM 510 INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 META Principle of Laser Scanning Microscopy Carl Zeiss INTRODUCTION TO LASER SCANNING MICROSCOPY Principle of Laser Scanning Microscopy To yield information on their inner structure by conventional transmitted-light microscopy, specimens have to be very thin and translucent; otherwise image definition will be poor. In many cases it is a problem to satisfy these requirements.

  • Page 44: Three-Dimensional Presentations Of Lsm Image Stacks

    Carl Zeiss Three-Dimensional Presentation of LSM Image Stacks LSM 510 META • In order to obtain an image of the selected object plane as a whole, it is necessary to scan the object plane in a point-by-point, line-by-line raster by means of an XY light deflection system. The detectors - as a rule, photomultipliers - convert the optical information into electric signals.
  • Page 45 LSM 510 INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 META Three-Dimensional Presentation of LSM Image Stacks Carl Zeiss • Color-coded height slices Each level, i.e. each slice is assigned a different color. For direct evaluation, a color scale is shown, indicating the actual height above the bottom slice.

  • Page 46: Optical Diagram Of The Lsm 510 (Schematic)

    INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 Carl Zeiss Optical Diagram of the LSM 510 LSM 510 META Optical Diagram of the LSM 510 (Schematic) λ- Fig. 3-2 Optical path, schematic (4-channel configuration) AOTF Acousto Optical Tunable Filter Dichroic Beam Combiner...

  • Page 47: Performance Features Of The Lsm 510

    LSM 510 INTRODUCTION TO LASER SCANNING MICROSCOPY LSM 510 META Performance Features of the LSM 510 Carl Zeiss Performance Features of the LSM 510 3.4.1 Optical and Mechanical Aspects The highly integrated system design makes for the shortest possible optical paths, top-grade optical precision and high stability.

  • Page 48: Microscope Equipment Of The Lsm 510 System

    3.4.2 Microscope Equipment of the LSM 510 System The LSM 510 system is equipped either with the inverted Axiovert 200 M BP or SP microscope or with the upright Axioplan 2 imaging , Axiotron 2 or Axioskop 2 FS mot microscopes.
  • Page 49 The side port or the front is equipped with the LSM 5 special interface, one of the others with the TV interface. The LSM 510 scanning module can be mounted to the special interface port. Different camera systems can be adapted to the TV interface using the TV adapters 452982/83/92/94/95/97/98-0000-000.

  • Page 50: Computer Hardware And Software

    The software of the LSM 510 has two levels. On the simple operator interface level, a result will be achieved after a few prompts; graphical prompting of the user in conjunction with automatic setting of many parameters is an ideal tool for daily routine jobs.
  • Page 51 LSM 510 QUICKSTART LSM 510 META Contents Carl Zeiss CHAPTER 4 QUICKSTART CONTENTS Page QUICKSTART........................4-3 Purpose of this Section and other Operating Manuals ............4-3 4.1.1 Software ..........................4-3 4.1.2 Windows and Window Elements..................4-4 4.1.3 Convention for the Text in this Manual.................4-5 4.1.4...
  • Page 52 QUICKSTART LSM 510 Carl Zeiss Contents LSM 510 META B 45-0008 e 10/02...

  • Page 53: Quickstart

    QUICKSTART Purpose of this Section and other Operating Manuals This section describes the operation of the LSM 510 and LSM 510 META Laser Scanning Microscopes exemplified by typical applications in conjunction with the LSM 5 software and its graphic user environment.

  • Page 54: Windows And Window Elements

    QUICKSTART LSM 510 Carl Zeiss Purpose of this Section and other Operating Manuals LSM 510 META 4.1.2 Windows and Window Elements Window element Description / Explanation Window (e.g.: Laser Control window) Window displayed after activation of a function − button (e.g.: Laser button in the toolbar of the Expert Mode).

  • Page 55: Convention For The Text In This Manual

    LSM 510 QUICKSTART LSM 510 META Purpose of this Section and other Operating Manuals Carl Zeiss Window element Description / Explanation Check box Activates / deactivates setting options. − Button Selection / performance of a function via mouse − click.

  • Page 56: Backup

    QUICKSTART LSM 510 Carl Zeiss Purpose of this Section and other Operating Manuals LSM 510 META 4.1.4 Backup System backup A complete backup is contained on the enclosed backup CD-ROM. − User files backup The following user-generated files need to be included in a backup procedure (keep directory structure): Image database files: *.mdb (but not system_configuration_*.mdb...

  • Page 57: Switching On The System

    LSM 510 QUICKSTART LSM 510 META Switching on the System Carl Zeiss Switching on the System The LSM system is turned on with the REMOTE CONTROL switch. This switches all the system components on except for the "Enterprise" UV laser.

  • Page 58: Log On To Windows Nt

    QUICKSTART LSM 510 Carl Zeiss Switching on the System LSM 510 META • Confirm the default setting of the "Windows NT Workstation Version 4.00" by pressing the Enter key. WINDOWS NT operating system is being − loaded. The Begin Logon window appears on the −...
  • Page 59 The WINDOWS NT operating system desktop appears on the screen, showing a number of icons. − Change Filters icon Stand Select icon LSM 510 icon LSM 510 Image Examiner icon LSM 510 Image Browser icon Fig. 4-5 WINDOWS NT operating system desktop B 45-0008 e 10/02...

  • Page 60: Switching On The Enterprise Uv Laser

    In that case, it is not necessary that the lasers and the microscope are switched on. • Double-click on the LSM 510 icon on the desktop of WINDOWS to start the LSM 5 software program (see Fig. 4-5). The LSM 510 Switchboard menu appears on the screen (see Fig. 4-8).
  • Page 61 Switching on the System Carl Zeiss Fig. 4-8 LSM 510 Switchboard menu The LSM 510 Switchboard menu presents the following items for selection: Scan New Images − Clicking on this button activates the complete LSM hardware (on-line mode). Use Existing Images −...
  • Page 62 QUICKSTART LSM 510 Carl Zeiss Switching on the System LSM 510 META Start Expert Mode − Use of this mode requires to be thoroughly familiar with the exact microscope procedures and interrelations. You need to set all parameters and functions upon your own decision; this mode therefore provides you with the greatest flexibility of operation.

  • Page 63: Quick Start In The Expert Mode

    − 4.3.1 Start the Expert Mode • Double-click on the LSM 510 icon on the WINDOWS NT operating system desktop. The LSM 510 Switchboard menu appears − on the screen.

  • Page 64: Set The Microscope

    QUICKSTART LSM 510 Carl Zeiss Quick Start in the Expert Mode LSM 510 META 4.3.2 Set the Microscope This step is used to set: microscope objective − specimen position − specimen focus − 4.3.2.1 Axioplan 2 imaging • Click on the Acquire button in the toolbar of the Main menu.

  • Page 65: Axiovert 200 M

    LSM 510 QUICKSTART LSM 510 META Quick Start in the Expert Mode Carl Zeiss 4.3.2.2 Axiovert 200 M • Click on the Acquire button in the toolbar of the Main menu. • Click on the VIS button in the Acquire subordinate toolbar.

  • Page 66: Turn On The Lasers

    QUICKSTART LSM 510 Carl Zeiss Quick Start in the Expert Mode LSM 510 META 4.3.3 Turn on the Lasers This step is used to switch the lasers and set the intensity. • Click on the Laser button in the Acquire subordinate toolbar of the Main menu.

  • Page 67: Set The Beam Path

    LSM 510 QUICKSTART LSM 510 META Quick Start in the Expert Mode Carl Zeiss 4.3.4 Set the Beam Path This step is used to specify beam path parameters by using a predefined Track Configuration. • Click on the Config button in the Acquire subordinate toolbar of the Main menu.

  • Page 68: Scan An Image

    QUICKSTART LSM 510 Carl Zeiss Quick Start in the Expert Mode LSM 510 META 4.3.5 Scan an Image This step is used to specify parameters and execute image acquisition. • Click on the Scan button in the Acquire subordinate toolbar of the Main menu.
  • Page 69 LSM 510 QUICKSTART LSM 510 META Quick Start in the Expert Mode Carl Zeiss • A specimen with 2 labels (FITC, Rhod.) with defined channels is easier to view in split screen where each channel is arranged side by side. You can toggle between xy and Split xy in the Image Display window.
  • Page 70 QUICKSTART LSM 510 Carl Zeiss Quick Start in the Expert Mode LSM 510 META Proceed as follows for image optimization: • Click on the Channels button in the Scan Control window. The Channel Settings panel and the − Excitation of Track (...) panel are displayed in the Scan Control window.
  • Page 71 LSM 510 QUICKSTART LSM 510 META Quick Start in the Expert Mode Carl Zeiss • To further improve image quality you can slow down the scan speed, allowing more photons to integrate on the detector, or apply image averaging to remove random noise, or a combination of both.

  • Page 72: Store The Image

    QUICKSTART LSM 510 Carl Zeiss Quick Start in the Expert Mode LSM 510 META 4.3.6 Store the Image This step is used to activate an existing database or to create a new database in which the acquired image is stored with the used settings and comments.
  • Page 73 LSM 510 QUICKSTART LSM 510 META Quick Start in the Expert Mode Carl Zeiss Proceed as follows to store images in a new database: • Click on the New MDB button in the Save Image and Parameter As window. The Create New Database window will be −...

  • Page 74: Shut-Down Procedure

    This closes the respective window and removes the respective icons from the taskbar. − After all dialog windows have been closed, the LSM 510 Switchboard window appears. − Fig. 4-23 LSM 510 Switchboard menu •...

  • Page 75: Shut Down The Windows Operating System

    LSM 510 QUICKSTART LSM 510 META Shut Down the Windows Operating System Carl Zeiss 4.4.2 Shut Down the WINDOWS Operating System • Move the cursor to the bottom margin of the screen. This opens the taskbar containing the Start button.

  • Page 76: Turning Power Off

    Power Supply switch of the Enterprise UV laser. Throw the REMOTE CONTROL main switch and the power supply switch of the Ar Laser to position "OFF" after 5 minutes. This puts your LSM 510 microscope system, including the computer, off power. − 4-26...

Table of Contents