Zeiss LSM 880 Operating Manual page 530

ZEISS Center Screen Area / Image Containers - Display and Image Analysis
For three of the terms (G
corresponding Settings button.
Fig. 740 Settings for the Scanning term
Fig. 741 Settings for the Amplitude term
524
CHAPTER 1 - SYSTEM OPERATION
, A and G ) settings menus are available. To open a settings menu, press the
s d
000000-2071-464
Scanning G (ξ,ψ) describes the scanning term of
s
the model function. Scanning is only active in the
Show all mode. To open the Scanning settings
press the Settings button (Fig. 740).
If the Diffusion coefficient check box is ticked,
the diffusion coefficient will be fitted, otherwise
the diffusion time. In case the box is checked, the
2 Photon check box will be accessible. If ticked
diffusion time scales with 1/8
coefficient, if un-ticked with 1/4
of the laser beam can be entered within the ω
µm input box by typing or using the arrows. Pixel
dwelling times and line times can be entered in the
Pixel time in µs and Line time in µs input boxes.
These values are useful for simulations when fixed.
They will be overwritten with the correct Meta
data from the image, whenever an image is
loaded. If these Meta data are not available, values
have to be provided.
information box the formulas for the Scanning
term G (ξ,ψ) and the conversion formula between
s
diffusion time t
and diffusion coefficient D are
d
displayed as well as a description of the parameter
variables.
Offset d describes any scalar added to the
correlation function. It will be necessary, if the
correlation function does not decay to zero.
Amplitude A describes number of particles taking
into account the form of the point spread function
in relation to the studied structure expressed in the
geometric correction factor γ. To open the
Amplitude settings press the Settings button
(Fig. 741). You can select a cylindrical PSF with
γ=1, a two dimensional Gaussian (2DG) PSF with
γ=0.5, a three dimensional Gaussian (3DG) PSF
with γ=0.35 or a Gaussian-Lorentzian (GL) PSF with
γ=0.076 by pressing the respective button.
Alternatively, if Calibrated is active, you can enter a
calibrated value via the Calibrated input box.
In the Amplitude information box the formulas
for the Amplitude term A is displayed as well as a
description of the parameter variables.
LSM 880
th
of the diffusion
th 2
. The 1/e radius
in
r
Scanning
In the
10/2014 V_01