Definition Of A Model - Zeiss LSM 880 Operating Manual

LSM 880
5.3.4.4

Definition of a Model

• To define a model press the Term tab of the term you want to be part of your final correlation
equation. The tab will be highlighted in blue. Press the tab again to remove the term from the final
equation. The tab becomes grey. You can choose from:
− Offset d: Deviation from 1
− Background B: Unspecific non-correlating background
− Amplitude A: Number of molecules and geometric factor
− Antibunching G
− Triplet G
(t): Exponential correlation terms for triplet state, blinking, flickering or other bunching
t
correlation terms
− Rotation G
(t): Rotational diffusion correlation terms
r
− Translation G
(t): Translational diffusion correlation terms
d
− Flow G
(t): Flow correlation terms
f
− Stretched exponential G
• All highlighted terms are assembled into the final equation that is displayed in G(t)= display area.
• For a selected term the Settings button becomes available. Press the Settings button to open the
Settings panel. Press the Settings button again to close it.
• The Settings panel allows you to choose specific equations and assign values to parameters, if
applicable. The Settings Description box provides information on the parameter or displays the
formula of the equation. In addition it gives useful conversions of the fitted parameter to other
interesting parameters.
The following settings are available:
Offset
• You can set the offset (Fig. 276) to 0 by
pressing the Normalized button.
• You can define an offset by pressing the
Calibrated button. This enables you to type in
a value in the Calibrated selection box, or by
setting a value using the arrows.
Note, the offset can have positive or
negative numbers and the value specified
will be added to all correlation values.
The Offset description box gives you information
on the offset.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
(t): Antibunching correlation term
a
(t): Stretched exponential correlation terms for kinetics
e
000000-2071-464
Fig. 276 Offset Settings panel
ZEISS
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