Zeiss LSM 880 Operating Manual page 272

ZEISS
Home and Load positions can also be accessed from the z-position slider by pressing the H or L buttons.
The z-position can be altered by grapping and sliding the plane or by using the arrow slider. The plane
shows the actual z-position.
Objective button: The objective can be selected via graphical pop-up menu. Please note, that for FCS
only the C-Apochromat 40x/60x W N1.2 is specified.
Reflector button: The reflector cube can be selected via graphical pop-up menu.
Tube Lens button: The tube lens can be selected via graphical pop-up menu.
HBO Reflected Light Open/Close Shutter button: The shutter for reflected epi-fluorescent light from
the HBO lamp is switched on and off by repeated pressing. The light path will be highlighted in color.
For transmitted-light observation
• Switch the HAL lamp on by the HAL On/Off button and adjust the light intensity.
• Activate the condenser function with the Condenser button.
• Press the Objective button to swing the required objective for FCS measurements into the working
position.
For Reflected-light Observation (Epi-fluorescence)
• Turn on the HBO power supply switch.
• Switch the HBO Reflected Light Open/Close Shutter into the Open position.
• Select the desired filter in the reflector turret by clicking the Reflector button. The filter will be
automatically moved into the beam path to enable observation in epi-fluorescence.
• Select the desired tube lens by clicking the Tube Lens button. Normally, the correct lens 1x will be
chosen automatically when the Locate tab of the main tool bar is pressed.
• Swing the required objective for FCS measurements into the working position by selecting the
objective with the Objective button. We recommend to use the C-Apochromat 40x/1.2 W corr
because of its optimized optics.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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LSM 880
10/2014 V_01