Coincidence - Zeiss LSM 880 Operating Manual

ZEISS
5.3.21.7

Coincidence

The coincidence analysis generates a two-dimensional color-coded count rate histogram (Fig. 374) in this
diagram. During the measurement the count rates in channel 1 (red) and channel 2 (blue) are registered
in small time windows and the respective frequencies are calculated. The count rate in channel 1 is than
plotted versus the count rate in channel 2. All values are incremented in the diagram and count rates
occurring simultaneously in both channels will populate the diagonal of the diagram.
All the table functions in the Coincidence mode are identical to those in the Correlation mode
The Coincidence diagram is currently only a display help and data cannot be exported. There are
no options available for the Coincidence diagrams. Coincidences can support analysis in case of
rare event detection of a standard cross-correlation experiment, when the measurement time drops
short for sufficient statistical significance, because it might be prohibitively long - as a rule of
thumb; approximately 1000 transitions of a labeled species are required to generate a correlation
curve of sufficient quality.
Fig. 374
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
Coincidence diagram panel
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LSM 880
10/2014 V_01