Zeiss LSM 880 Operating Manual page 504

ZEISS Center Screen Area / Image Containers - Display and Image Analysis
Fig. 690 Save to Spectra Database: database interface with subfolder structure
498
CHAPTER 1 - SYSTEM OPERATION
An additional channel per unmixed component is displayed which
shows the relative standard deviation (RSD) in each unmixed component
channel (see also section Processing – Linear Unmixing).
A separate channel in the unmixed image that displays the difference
between fit and original data in grey values for the channel of the
Lambda stack that shows the greatest deviation (see also section
Processing – Linear Unmixing).
The values of the intensity Lambda diagram are saved to the spectra
database in the ZEN\DyeDatabase directory. Make sure to have
displayed only data of single dyes in the intensity Lambda diagram when
executing this function. (The database interface is shown in Fig. 690).
Auto Find / ACE ("Automatic Component Extraction")
Automatically searches the image for regions with pure signals
(Fluorescence originating from one single dye).
Performs the unmixing with the selected spectra. Note: Those channels
of the Lambda stack which are de-selected in the dimensions control
tab are not included in the unmixing calculation.
000000-2071-464
LSM 880
10/2014 V_01