Zeiss LSM 880 Operating Manual page 113

LSM 880
Frame Mode for LSM
When the scan mode Frame is active, a frame of
variable size is scanned pixel by pixel and line by
line. The laser beam is moved over the specimen
line by line.
The following additional parameters and options
can be set:
Objective, Frame Size, Line Step Factor, Speed,
Bit Depth, Scan Direction, Averaging and
Zoom including rotation and offset
A list of available objectives is displayed in the
scroll down menu next to Objective. Choose one
from the list which is appropriate for imaging a
specific specimen. Pay attention to match the
refractive index of the objective with the refractive
index of the embedding medium of the specimen
if possible.
• Select the Frame Size from the default sizes via
the drop down menu clicking the
or type in the required values next to X and Y.
Recommended setting to start with: 512 x 512
pixels.
− It is also possible to enter different values for
X and Y. The value for Y is freely selectable
between 1 and 8192 pixels (integers). The
value for X must always be an integral
multiple of 4 and will be corrected if not
typed in already. The maximum value for X is
also 8192 pixels.
− The Optimal
resolution to an optimal value corresponding
to the optical magnification (objective) the
zoom and the emission range detected. This
provides an image where no information is
lost as optimal sampling is achieved. The
optimal value is calculated for the give
objective and zoom settings matching a
2fold oversampling according to 2 fold
Nyquist.
• Select the Line Step size between 1 and 10.
Only every n-th line is scanned. The lines in
between are interpolated. This fast scan mode is
called Step Scan.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
button
button sets the image
000000-2071-464
Fig. 135 Acquisition Mode - Frame with LSM
tab selected in the Imaging Setup
tool
ZEISS
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