Zeiss LSM 880 Operating Manual page 197

LSM 880
• Set the Measure Time.
If you do not have any idea about the behavior of your sample, start with a measurement time of
about 10 seconds. Depending on the signal-to-noise ratio of your correlation curve, decrease or
increase the measurement time.
• Set the Repetitions.
Start with Repetitions set to 1. If you want to obtain information about the variation of your fitted
values later on, increase the repeat count to produce a measurement series. 10 times is a good value
to start with.
The total duration of the measurement will be indicated as Calculated duration in the Time
Series tab.
• Click on the Acquisition tab to open the Acquisition panel (Fig. 271). Click on any other tab to leave
the panel.
In the Channel area you can assign from a look up table a color for each channel. Every channel selected
under Light Path tab will be displayed. With BiG.2, NDD and the 3 Channel Module you have up to four
channels, two auto- and two cross-correlation channels. With the 32 Channel Module you have up to
eight auto-correlation channels and accordingly 8 faculty cross-correlation channels.
In the Lasers area you can activate any available laser by checking the corresponding box and set the
attenuation power. The attenuation power will be updated between the Acquisition tab and Light Path
tab. The set laser power will be used for the measurement and pinhole alignment.
In the Pinhole area the Pupil filling slider controls an optional iris in the excitation beam that allows you
to enlarge the size of the confocal volume by decreasing the pupil filling. Only if present will the Pupil
filling slider and input box be available in the Pinhole menu. You can enter a value by typing, using the
arrows or the slider. The Pinhole slider allows you to et the diameter of the pinhole. The Airy units are
displayed right of the Diameter slider. By pressing the 1 Airy button the pinhole diameter will be set to 1
Airy unit for one selected laser line, or a compromise between all selected laser lines.
Note, if the system is switched to LSM mode, the pupil filling will automatically be set to 100 %.
The last entered value will be taken when switching back to FCS mode.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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ZEISS
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