Zeiss LSM 880 Operating Manual page 508

CHAPTER 1 - SYSTEM OPERATION
ZEISS Center Screen Area / Image Containers - Display and Image Analysis LSM 880
FRETN (p): Result of analysis using Gordon method. The FRETN value is calculated for each pixel,
afterwards the average for the chosen region is determined.
Fc (p): Result of analysis using Youvan method. The Fc value is calculated for each pixel, afterwards the
average for the chosen region is determined.
N-FRET (p): Result of analysis using Xia method. The N-FRET value is calculated for each pixel, afterwards
the average for the chosen region is determined.
Fig. 694 Table after Acceptor Photobleaching analysis
After an Acceptor Photobleaching analysis the table displays the following parameters (Fig. 694):
Region: The identification number of regions assigned for analysis. Region zero refers to the whole
image.
FRET(p) Eff. %: FRET efficiency for each pixel is calculated and averaged for all pixels of the region.
FRET Eff. %: The averaged intensities of the region are used to calculate an averaged FRET efficiency for
this region. Delta D / D Post * 100.
D Pre: Average donor intensity of the region in the pre-bleach image.
D Post: Average donor intensity of the region in the post-bleach image.
A Pre: Average acceptor intensity of the region in the pre-bleach image.
A Post: Average acceptor intensity of the region in the post-bleach image.
Delta D: Change in donor intensity of the region before and after the bleach event.
Delta D = D Post-D Pre.
Delta A: Change in acceptor intensity of the region before and after the bleach event.
Delta A = A Post- A Pre.
Independent of the FRET method, the FRET View Options Control Block offers four tabs:
The available functions within each tab can vary according to the image loaded.
It is assumed that a time series with bleaching represents an experiment with Acceptor Photobleaching
and a three channel image represents an experiment performing Sensitized Emission.
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