Zeiss LSM 880 Operating Manual page 142

ZEISS
• If the Automatic Movement to next Position is activated, the stage will move automatically to the
next marker (Position 2) after clicking Accept. Otherwise, the marker 2 has to be found manually.
Fig. 181 Sample Holder Calibration field,
Stage movement to next number
• The system will propose the position for marker 2 in a Marker location found dialog. Confirm the
proposal if the position is correct.
The stage moves automatically to marker 3.
• Click on Find Marker in the Position 3 field and confirm the position if correct.
The calibration is finished. The calibration can be started by clicking on Restart Calibration in the
Shuttle and Find tool window.
Fig. 182 Positions button in the Dimensions
control tab of the 2D view
• Activate the Positions button in the Dimensions control tab (Fig. 182).
• Click either on the calibration marker or a user-defined marker to set a crosshair in the image and thus
define the position of the marker.
• Confirm the position by clicking on Manual in the Set Marker Positon section within the Shuttle
and Find tool.
• If the Automatic movement to next marker checkbox is not activated, move the stage to the
second marker and got on as described above.
• Repeat the same procedure for the third marker.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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• If the stage is moving in the wrong direction,
invert the direction by clicking on X Invert or Y
Invert in the Sample Holder Calibration field
(Fig. 181).
• When marker 2 is in position refocus and click
on Find Marker in the Position 2 field.
b) Manual marker recognition
If the marker on the sample holder is not
recognized or user-defined markers are used, the
marker positions can be set manually.
• Activate the Acquisition Parameter Positions
within the Acquisition Parameter list.
• Click on the Continuous button to start
continuous scanning.
• Move the stage to the first marker.
• Click on Snap to acquire a single image.
LSM 880
10/2014 V_01