Fcs Correlation Diagram - Zeiss LSM 880 Operating Manual

ZEISS
The Image FCS Diagram consists of four data layers:
− FCS Correlation
− FCS Coincidence
− FCS Fit
− FCS Print
Each layer will display the data as they were stored last. As default, the first layer FCS Correlation will be
displayed. Select other layers by pressing the corresponding tabs.
If a file format is loaded that is not recognized by the FCS software, an empty Image FCS Diagram
will open.
If a corrupted .fcs or .raw data file is loaded, an empty Image FCS Diagram will be loaded.
If a .fcs data file is associated with .raw data files, raw data files will be loaded in the Image FCS
Diagram as well.
5.3.21.3

FCS Correlation Diagram

• Click on the FCS Correlation tab to activate the FCS Correlation display mode (Fig. 359).
The FCS Correlation Diagrams display graphic curves including Count Rate, Correlation Function
G(t), Pulse Density Histogram (Photon Counting Histogram) and Pulse Distance Histogram. It also
displays the Result Table. A scrollbar at the bottom of the diagram allows to view the content of the
displayed window in case it is to small to show all data within the container size.
Choose the single diagrams you want to display by activating the corresponding buttons in Diagrams of
the Options / Correlation Diagrams field. Selected diagrams will be highlighted in blue. By clicking on
the appropriate button, the diagram can be toggled between ON and OFF.
The FCS Correlation Diagram can be expanded horizontally and vertically by closing the File Handling
Area and by pressing the downward pointing arrowhead beneath the Table scrollbar. The arrowhead will
point upwards and pressing it again will restrict the vertical extension of the diagram again. The size of
the single diagrams and the table can be matched as required by moving the border lines. Click on the
border line, hold down the mouse button, move the line in the required direction and release the mouse
button.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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LSM 880
10/2014 V_01