Zeiss LSM 880 Operating Manual page 151

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
Pressing the Center button will define the current focus position (as defined in the Position (µm) input
box) as the center position of the stack. After pressing Center the whole range will move by the
difference between of the previous center position and the new one. The number in the Center input
box will then equal the number in the Position (µm) input box.
The Offset input box allows to define an offset between the actual focus position as displayed in the
Position (µm) input box and the current center position as displayed in the Center input box. One can
edit the number by typing or using the arrow keys. When defining a specific Offset the Center position
will be recalculated as the actual position plus the offset. Accordingly the range of the Z-Stack is shifted
to a different position in Z. Pressing the C button of the Z Navigation representation moves the actual
Focus Position to the Center position and acquires an image at that position. A Z-Stack is then acquired
at the new position when starting the experiment. Hitting Center moves the Center position back to the
actual Focus Position setting the Offset again to zero.
Use the F, C and L buttons to get images from the first slice, center slice and last slice of the stack. The
Focus Position updates accordingly. The Offset value then might be set to half of the Interval
depending on the interval value and the number of slices, as with a lower number of slices or a large
interval the real center position of the stack might be just between two slices.
Pressing the Range Select button will start a XZ-scan within the Range of the Z-Stack. This helps setting
the Range of the Z-Stack without continuously scanning complete frames. The position of the first and
last slice is indicated with a red line, the Focus position, which initially corresponds to the Center
position, is indicated as a green line. Changing the Offset value moves the red lines in parallel to the
new offset position, the green line, while the Focus Position is kept constant. Pressing the C button sets
the Focus Position to the indicated Center position and the Offset back to zero. In parallel an image at
the newly defined Focus Position is acquired and displayed. Alternatively, pressing Center sets the
indicated Center Position back to the actual Focus Position moving the red lines back to the original
position and setting the Offset back to Zero. The original values for Number of Slices, Interval and
Range are always kept constant. The red lines can be moved independently from each other allowing an
optimal adjustment of the Z-Stack range to the specimen. Center sets the green line to the center
position between the red lines and adjusts the Focus Position accordingly. The original values for Number
of Slices and Range are adjusted. The Interval is kept constant.
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