Focus Control; Hal Control - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 103 Focus control – expanded view
Pressing Manually in the Zero position field will set the current z-position to 0.
If a Piezo stage insert is in use and active then pressing Levelling in the Piezo field will center the Piezo
stage position at the current focus position of the microscope focus drive.
The Piezo Position (µm) input box allows the position of the Piezo to be altered. The Step Size (µm)
display box shows the currently used step size for focus movement of the Piezo stage.
The Scroll bar allows accessing the whole Focus control panel.
Fig. 104

HAL control

88
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
000000-2071-464
5.2.7.5

Focus Control

If the Focus button is pressed, the Focus control
panel will appear (Fig. 103). The Z-Position display
box shows the current z-position. The number can
be edited by typing or using the arrowheads. The
nosepiece or stage will automatically update the
new position.
By pressing the Stop button, you can stop the
current movement.
The Step Size (µm) display box shows the
currently used step size for focus movement. The
value can be edited by typing or using the
arrowheads.
The Home button sets the z-position to the
predefined zero value. Pressing Load will lower the
nose piece (z-position) for objective change.
Work brings the z-position back to the actual used
position if the focus was zeroed or in Load
position. Home and Load positions can also be
accessed from the z-position slider by pressing the
H or L buttons. The z-position can be altered by
grapping and sliding the plane or by using the
arrow slider. The plane shows the actual z-
position.
5.2.7.6 HAL Control
When the HAL On/Off button (only available for
WF imaging mode) for transmitted light of the
halogen lamp is pressed, the control panel for the
light settings is displayed (Fig. 104).
If you press the On button, the light intensity of
the HAL lamp can be varied via the input box or
slider. The light path will be highlighted by color in
the diagram. Press the On button again to shut
down the light.
LSM 880
10/2014 V_01