Principle Of Airyscanning - Zeiss LSM 880 Operating Manual

CHAPTER 1 - SYSTEM OPERATION
ZEISS Hardware Aspects LSM 880
2.3

Principle of Airyscanning

The Airyscan detector consists of 32 elements based on GaAsP PMT technology with each element
detecting the emission signal of a focal spot much smaller than the typical 1 Airy volume.
This detection scheme together with adapted deconvolution algorigthms allows resolving fluorescently
labelled structures to a much higher degree. The resolution reaches 140 nm in x and y and 400 nm along
the optical axis.
The emission signal is filtered using standard emission filters.
The detected signal is processed online for immeditate judgement of the resulting 2D image. As the
detector works along the confocal principle an increase in resolution is achieved in 3D and using standard
specimens prepared for confocal imaging.
Another detection mode provides the ability to virtually open or close the pinhole after the image has
been acquired. In addition the detector can be used as an additional confocal detector and in
combination with other detectors of the system.
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