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Zeiss LSM800-2 Quick Start Manual

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!!! IMPORTANT !!!
Please make sure that the chosen objective
corresponds to the one shown in the software.
If this is not the case, please restart the system.
(Otherwise the pinhole and pixel sizes, as well as the
sectioning thicknesses are all incorrect)
!!! IMPORTANT !!!
LSM800 upright has two new GaAsP PMT detectors.
As already explained during intros, the new PMT are very
sensitive and easy to oversaturate (and damage).
GaAsP detectors are shutting down if oversaturated,
terminating your experiment without further warnings!
Please use them correctly!

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Summary of Contents for Zeiss LSM800-2

  • Page 1 !!! IMPORTANT !!! Please make sure that the chosen objective corresponds to the one shown in the software. If this is not the case, please restart the system. (Otherwise the pinhole and pixel sizes, as well as the sectioning thicknesses are all incorrect) !!! IMPORTANT !!! LSM800 upright has two new GaAsP PMT detectors.
  • Page 2 Zeiss LSM800-2 Upright confocal microscope August 6, 2019 System description:  Upright confocal microscope ZEN blue 2.6/ Hotfix-3  Motorized stage for multiple position imaging  Heating chamber  NO CO mixer  Multislide holder, dish holder, single slide holder...
  • Page 3 7. Login: LSMuser (password: Useme!11) 8. Open ZEN Blue Software and press “ZEN System” 9. “Image Processing” is for offline analysis of your images, please do not use it here III. Visual examination of the sample 1. Put mechanical switch on the right hand side of the microscope to the ”Eye” position. 2.
  • Page 4 Note: Acquiring more pixels than ”optimal” almost never makes sense, but feel free to decrease the pixel number to increase acquisition speed at the cost of resolution. 10. To capture single images, click at ‘Snap’. 11. Setting up Z-stack: Enable Z-Stack and the Z-Stack dialog will appear Press “Live”...
  • Page 5 5. Switch off COMPONENTS (3). 6. Switch off SYSTEM (2). 7. Switch off multiple sockets (1).

  • Page 6: Laser Safety Instructions

    Laser Safety Instructions  During operation of class 3B and class 4 lasers red warning lights have to be switched on manually  Red warning light at the door of the room, containing laser-based equipment, prohibits the entrance  Optical path of the laser beam at all setups has to stay intact and should never be disassembled by a user. Users are never permitted to disconnect optical connections (pipes, fibers etc), remove protective coverings or disassemble any parts of the setups, especially those parts that are labeled with laser-warning signs.