Zeiss LSM 880 Operating Manual page 556

ZEISS
Sample carrier ......................................... 229
Stage ..................................................... 228
NDD light path ............................................ 181
Nearest neighbour ....................................... 364
New image document button ........................ 48
O
Objective ..................................................... 402
Objective selection ................................ 86, 101
Offline calibration ........................................ 315
Offset ......................................................... 213
Offset values ............................................... 166
Online multiview processing ........................ 381
Online ratio ................................................. 316
Optical diagram ............................................. 13
Options ....................................................... 409
Options tools
Correlation tab ....................................... 271
Table tab ................................................ 273
Ortho
Distance ................................................. 445
Select ..................................................... 444
Overview image .................................. 154, 167
P
PALM .......................................................... 326
Convert to image .................................... 327
Import PALM molecules .......................... 328
PALM ..................................................... 330
Remove PALM outliers ............................ 338
Parfocal correction ...................................... 406
Particle tracking ........................................... 339
PCH .................................................... 222, 226
Photon count images................................... 121
Photon count imaging ................................. 121
Photon counting histogram ......................... 277
Piezo ........................................................... 146
Pinhole adjustment ....................... 186, 396, 399
Polarization imaging .................................... 527
Position list .......................................... 162, 243
Positions ............................................. 162, 238
Pre-Registration ........................................... 372
Print ............................................................ 290
Processing ................................................... 293
Adjust .................................................... 391
Airyscan Processing ................................. 297
Average .................................................. 304
Channel alignment ................................. 354
Color-coded projection ........................... 301
Copy ...................................................... 382
Correlation ............................................. 319
Experimental PSF .................................... 341
Filter ....................................................... 305
550
CHAPTER 1 - SYSTEM OPERATION
Index
Profile .......................................................... 476
Pulse distance histogram .............................. 277
R
Range indicator ............................................ 425
Ratio channels ............................................. 120
Ratio imaging ................................................90
Ratiometric dyes .......................................... 316
Ratiometric dyes - Calibration ....................... 317
Ratiometric dyes - Equation calibration ......... 318
Ratiometric dyes - Online ratio ...................... 316
Raw data formats......................................... 414
Reference spectra......................................... 312
Reflected-light observation ..................... 57, 266
Registration ......................................... 368, 372
Registration options ..................................... 368
Regularized inverse ...................................... 364
Reload ......................................................... 272
Remove structure ......................................... 385
Reuse .................................................. 272, 426
RICS ............................................................ 508
Right tool area ...............................................48
Rotation ...................................................... 217
S
Sample carrier .............................. 163, 229, 240
Save button ...................................................48
Save status icon .............................................49
Saving ......................................................... 278
Saving an image .......................................... 545
Scan area ..................................................... 105
Scan mode................................................... 102
Scatter diagram ........................................... 474
Sectioning.................................................... 147
Separate view controls ...................................47
Series ........................................................... 463
Set Exposure ................................................ 171
Settings ....................................................... 466
Setup Imaging with Airyscan detector .............99
Setup tools
000000-2071-464
HDR-imaging ........................................... 321
ICS .......................................................... 384
Image calculator ...................................... 302
Ion concentration .................................... 313
Lightsheet processing .............................. 361
Linear unmixing ....................................... 308
Maximum intensity projection .................. 300
Modify series ........................................... 320
PALM ...................................................... 326
Particle tracking ....................................... 339
SIM ......................................................... 345
Stitch ...................................................... 325
Structured illumination ............................ 345
LSM 880
10/2014 V_01