Zeiss LSM 880 Operating Manual page 603

LSM 880
CHAPTER 4
CONTENTS
1 Software and Hardware Options ......................................................................... 2
1.1 Software ............................................................................................................................ 2
1.2 Hardware ........................................................................................................................... 4
2 Courses on "How to Operate the System in an Optimized Way"...................... 5
3 Beam Path Configuration Guide for Fluorescence Microscopy .......................... 5
3.1 Optical Elements in the Configuration Control Window ....................................................... 5
3.1.1 Main Dichroic Beam Splitter (MBS) ...................................................................................... 5
3.2 Setup of Tracks Using Single Detectors (Channel Mode) ....................................................... 6
3.3 Beam Path Configuration for Multi Photon Excitation .......................................................... 8
3.4 Acquisition Conditions for RICS ........................................................................................... 8
4 Recommendations for Excitation Laser Lines and Emission Filters of Dyes ...... 9
5 How to Access Data Acquired with Airyscan Using Other Software .............. 10
6 Piezo Focusing Device ......................................................................................... 11
7 Specification of Trigger-Interface ...................................................................... 13
8 Level-adjustable Sample Holder with Insert for Piezo Stage (ELYRA) ............. 18
8.1 Stage Leveling Using X-Cite or Laser in Fluorescence .......................................................... 18
8.2 Stage Leveling Using X-Cite in Reflection ........................................................................... 19
9 Level-adjustable Sample Holder for LSM .......................................................... 21
9.1 Stage Leveling Using LSM in Reflection Mode and Focus Drive ........................................... 21
9.2 Stage Leveling Using LSM in Reflection Mode and Z-Piezo ................................................. 22
10 Liquid cooling system of the LSM ...................................................................... 24
11 Detector overload protection ............................................................................. 24
10/2014 V_01
CHAPTER 4 - ANNEX
Contents
ANNEX
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ZEISS
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