Zeiss LSM 880 Operating Manual page 159

LSM 880
Tick the checkbox to activate online stitching. After
ticking the checkbox the Threshold input box
becomes available. Enter the desired threshold
in %.
By pressing Add you can mark stage positions,
which will be listed in the Marked position field
in terms of Number as well as x [µm] and y [µm]
coordinates (Fig. 206). By Pressing Remove the
highlighted position will be removed from the list.
Pressing Remove all will delete all positions. You
can save a position list by Pressing the Save...
button in the Mark position field via the
Windows Explorer. You can load saved position list
by pressing the Load... button via selection in
Windows explorer.
If Centered grid is selected, the current position
will be the center of the Tile Scan.
If Bounding grid is selected positions that should
be part of the tile scan have to be marked using
Add. To this end click on the edge of the square to
select the position, which will then be displayed
with a thicker line width, and then press Add.
Using these positions a bounding grid is created,
which finally defines the dimensions of the tile
scan (Fig. 207).
If Convex hull is selected positions that should be
part of the tile scan have to be marked using Add.
To this end click on the edge of the square to
select the position, which will then be displayed
with a thicker line width, and then press Add.
Using these positions a convex hull is created,
which finally defines the dimensions of the tile
scan (Fig. 208).
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 207 Tile Scan tool – Bounding grid
selected; two positions defined
Fig. 208 Tile Scan tool– Convex hull selected
ZEISS
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