Zeiss LSM 880 Operating Manual page 241

LSM 880
• Continue moving upwards slowly. The line
position will reach its maximum (Fig. 311).
• Having slowly moved over a short distance the
line position will decline again to a minimum
and than rise again to a second maximum
(Fig. 312), which corresponds to the upper
cover slip surface.
• Store the reached position by using the Store
button.
• Now you can position the detection volume into
the sample in a well-defined way:
If you use one of the C-Apochromat water
immersion objectives, position the detection
volume 150 - 200 µm deep into the sample to
get rid of disturbing interface effects. The
easiest way of doing this is by clicking the
+200 µm button.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
000000-2071-464
Fig. 311 Line diagram at lower cover slip
surface
Fig. 312 Line diagram at upper cover slip
surface
ZEISS
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