Zeiss LSM 880 Operating Manual page 320

ZEISS
Image scaling
Pull down menus for
Fig. 416 Fully opened Ion
Concentration tool window -
Ratiometric dye in equation
mode
314
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
Spin boxes to set the Min and Max in the Output Image
To set three different calibration options, according to the dyes (single
wavelength, vs ratiometric), the calibration method and if the calibration is
carried out in vitro/situ. Ch 1 and 2 select the channels from Input Image 1.
From these pull downs, the following combinations of
parameters can be chosen:
Dye: single wavelength vs. ratiometric
Available methods for single wavelength: Titration or
equation (both, in vitro and in situ calibration).
Available
titration, equation, and unmixing.
000000-2071-464
ratiometric
methods for
LSM 880
Ratio,
dyes:
10/2014 V_01