Image Optimization; Requirements - Zeiss LSM 880 Operating Manual

LSM 880
5.2.23

Image Optimization

This section describes an example how to acquire
an image, using an excitation wavelength of
561 nm and a fluorescence emission range above
570 nm. Use the MBS 488/561 as the main
dichroic beam splitter. We presume that no
predefined configuration is available for this
imaging task.
The description applies to the use of any
microscope.
Data acquisitions
keeping the computer busy with other
tasks then acquisition like processing data
or using other software in the mean time.
This might slow down image acquisition
and can even interfere with the timing of
the acquisition.
5.2.23.1

Requirements

• Position the specimen under the objective and
focus onto the desired imaging plane.
• Activate Show all Tools and open the
Imaging Setup tool. Chose Channel mode for
the image acquisition (Fig. 249).
• Switch on the Laser 561 nm.
• Click on the laser icon, select the 561 line, and
set the transmission slider to about 3 % (this
laser has an output power of 10 mW, other
lasers have up to 100 mW depending on the
type of laser and the imaging device used; be
careful when setting the power for imaging
with other lasers for imaging other dyes). Make
sure other lasers are not checked (Fig. 250).
Optimize the laser intensity subsequently
via the slider when imaging.
• Activate channel 1 (Ch1) via the check box
(Fig. 249).
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
is impaired when
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Fig. 249 Imaging Setup – imaging mode
selection and track activation
Fig. 250 Laser line and laser power selection
ZEISS
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