Zeiss LSM 880 Quick Operation Manual

Instruction LSM880 short 2017-12-26.docx 2017-12-26
Switch on
- Make sure room temperature is 22-24°C.
- If necessary turn on air condition
In the main switch box turn on the following switches 1  2  3 (do not touch the key)
-
- Turn on the computer and log in
User name: multilabs
Password: 123456
Position Objective lens
2
Plan Apochromat
3
Plan Apochromat
4 (bombila)
W Plan Apochromat
1+ adaptor
W Plan Apochromat
1+ adaptor
Plan Apochromat
Software and acquisition
-
Log into BookitLab and activate your reservation to start Black Zen 2.3
-
To acquire images click Start System
In the acquisition tab  Laser tab
-
Switch on the lasers, (The Argon laser should be turned on by Standby  ON. Please wait 5' minutes until
the laser stabilizes, its status should be optimal in the properties tab).
- Sample mounting and viewing
Insert the slide or dish in the holder.
To change objective turn the black wheel to point up to release the objective turret. Turn manually to the
required objective, lower the objective by pointing the black wheel in the objective turret down, (if an
objective needs to be replaced please ask the microscopy staff).
In the software go to Locate tab.
On the microscope's right side choose VIS
- Transmitted light
Click the transmitted light lamp icon, click ON and set desired brightness (or via the microscope).
Set the filter turret position to DIC and the condenser below the stage to II or III according to the objective
lens (see objective lens info on touchpad).
Set up Kohler illumination.
- Reflected light (fluorescence)
Open fluorescence shutter
Set the filter turret to the desired position
- Image Acquisition
On the microscope's right side choose LSM
In Zen, Acquisition tab:
Load a saved configuration from the Experiment Manager or open an image and below the image click
Reuse
Select Show all tools and open the following tabs:
LSM 880 quick operation manual
Magni-
NA coverslip
fication
x10 0.45 0.17 mm
x20 0.8 0.17 mm
x20 1.0
x40 1.0
x63 1.4
Working
distance
2 mm
0.55 mm
0 1.8 mm
0 2.5 mm
0.17 0.19 mm
Immersion Parfocal length
45 mm
Air
45 mm
Air
75 mm
Water dipping
45 mm
Water dipping
45 mm
Oil