Zeiss LSM 880 Operating Manual page 240

ZEISS
Fig. 308 Selection of Line Scan diagram
Fig. 309 Selection of Line Scan diagram
Fig. 310 Line diagram near the lower cover
slip surface
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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• Press Continuous to start a continuous scan.
Press the Line Scan tab (Fig. 308).
• The diagram (Fig. 309) shows a line with a
bump, which is the result of reflected light on
the optical axis not focused yet on the lower
glass surface You might adjust the line to lower
or higher values by altering the gain or laser
power.
• Move the objective cautiously upward by
turning the Z focus knob of the microscope
stand. A lens moving upward will be indicated
by increasing numbers in the position field of
the Z (Focus) panel window.
• If the focus position approaches the lower cover
slip surface, the position of the line will shift to
higher values (Fig. 310).
LSM 880
10/2014 V_01