Zeiss LSM 880 Operating Manual page 117

LSM 880
Line Mode
In Line mode, fluorescent or reflected light along a
straight or freely definable line is displayed in the
form of an intensity profile. The line is scanned
pixel by pixel. The laser beam is moved over the
specimen along a line.
The following parameters for scanning a line can
be defined:
Objective, Frame Size, Speed, Bit Depth, Scan
Direction, Averaging and Zoom including
rotation and offset
These functions operate as described in section
Frame Mode for LSM. Frame size in this context
sets the length of the line in x direction. The width
of the line is one pixel.
Note: A width larger than one pixel is defined as a
frame.
If Line Mode has been selected, the Line Select
button appears on the bottom of the Acquisition
mode tool. It permits positioning of the line to be
scanned as required within the Image (Frame in
XY-plane).
A frame will be scanned and the currently selected
scan line and its intensity profile will be displayed.
In the View Control the Line–select tools are
available.
The line arrow is active by default and allows using the mouse to draw a straight line in the image at
the position where the line scan should be performed.
Alternatively the scan line
The arrow selection
The standard controls allow defining the color and width of the appearance of the line overlay in the
image.
Once a straight line is drawn in the image, the relevant intensity profile along the drawn line will be
displayed. In the Acquisition Mode tool Zoom, Rotation and Offset values are updated.
• When the Line Select button is hit again, a frame will be scanned in such a way that the selected line
lies exactly in the center of the Y-axis again and is parallel to the X-axis.
The position and size of the Line (rotation and offset) can also be changed directly in the Scan
Area panel of the Acquisition Mode tool.
In the Line mode, Line Stacks can also be recorded over a defined period of time.
• When drawing a free shape curve (spline) in the Image using the mouse, the first click sets the
starting point; each further click adds a line segment. A click with the right mouse button ends the
line definition.
The scanner represented by a white line immediately begins with the on-line tracing of the defined free
shape curve. The laser excitation remains inactive in this process.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
can be defined as a free shape curve (spline).
is used to move the drawn line within the image.
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Fig. 138 Acquisition Mode – Line with LSM
tab selected in the Imaging Setup
tool
ZEISS
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