Zeiss LSM 880 Operating Manual page 145

LSM 880
The following tools are available in the Tool selection field:
Selects an existing region in the image. The outline of the region is marked with
small rectangles to show it is active. The format and the position of the region can be
changed clicking on the rectangles or the region outline and dragging it with the
cursor. In addition the numerical values describing the position and size of the region
are updated.
Rectangle: Drawing of a rectangle in the Image Display window; click and keep
mouse button pressed, drag the rectangle in any direction, let go off the mouse
button to end the procedure.
Ellipse: Drawing of an ellipse in the Image Display window; first click sets the
center point, displayed line permits determination of the extension, second click sets
the first dimension, then the second dimension and the rotation direction can be
determined, third click sets the second dimension and direction and ends the
procedure.
Circle: Drawing of a circle in the Image Display window; click and keep the mouse
button pressed to set the center point, drag the diameter, let go off mouse button
again to end the procedure.
Polyline: Drawing of a polyline figure in the Image Display window; first click sets
the starting point, each further click adds a line, double-click on the starting point
closes the figure and ends the procedure.
Closed Bezier: Drawing of a bezier figure in the Image Display window; first click
sets the starting point, each additional click adds a line, double-click on the starting
point closes the figure and ends the procedure.
By pressing the Delete button, the highlighted
region will be removed. By ticking the Hide
checkbox, the region will be hidden in the image.
All drawn regions will be listed in the Region list
field. They are identified by an assigned number
and its form.
The task (Acquisition, Bleaching, Analysis) for
which a region will be used can be defined by
ticking the respective boxes (Note that Bleaching
is only useful in the LSM imaging mode).
E.g., if Acquisition is checked, only the defined
region is illuminated (LSM imaging mode only)
and displayed (Fig. 188). Any combination of these
tasks is possible.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 188 Image with two defined regions
used for imaging
ZEISS
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