Zeiss LSM 880 Operating Manual page 236

ZEISS
Fig. 302 Delete configuration window
Fig. 303 Delete confirmation dialog
• If you want to edit a pre-existing carrier or want to create a new one press the Properties button
open the Properties window.
• Enter the number of chambers and the distance (mm) between the chambers in the appropriate
Column and Row input boxes.
• To orient the sample carrier in x and y click on the Calibrate.
• Position the center of one chamber over the center of the objective by clicking the arrow keys of the
compass rose with the mouse or by using the control panel's joystick (if available).
• Click on the corresponding chamber in the Orientation window.
− This chamber will be highlighted. When you have finished the Calibrate deactivates automatically.
The stage is now oriented.
Now you can move any chamber into the measurement position by clicking the appropriate field on the
sample carrier.
− The selected chamber is then automatically approached via the motorized microscope stage. When
the defined position has been reached, the position of the chamber is displayed in the lower cleft
corner in the Positions tool and is highlighted in blue.
By pressing the Help button (? question mark icon), a Help window appears explaining the work
flow for orienting the carrier.
If the motorized stage is moved, calibration is lost.
If you move from well to well without having calibrated the system, the system might overshoot
the selected well position.
The sample carrier window is only active if a motorized stage is attached to the system. No sample
carrier will be displayed, if a manual stage is used.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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In order to delete a configuration, press the Delete
button (cross symbol). This will open the Delete
configuration... window (Fig. 302).
Select the configuration you want to delete by
clicking on its name. You will be asked in the
Delete confirmation dialog, if you want to delete
the configuration (Fig. 303). Press Yes to delete
the configuration, No to close the window without
deleting. If you enter a name in the Search field,
only this configuration will be displayed in the
Configurations box.
If the typed name has no match in the
configurations list, this will remain empty.
To calibrate the sample carrier, press the Calibrate
button, which will stay active until pressed again or
a well position was selected.
Procedure for selection and calibration of a
sample carrier
From the Load button dropdown menu box select
a pre-defined carrier. This will display the sample
carrier in the Orientation box.
LSM 880
10/2014 V_01