Zeiss LSM 880 Operating Manual page 120

ZEISS
Fig. 142 Airyscan: ILEX Setup activation
Fig. 143 Airyscan: ILEX Setup panel
Fig. 144 Airyscan: Channels settings
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Airyscan Multitrack ILEX
For best synchronicity in a multitracking acquisition
with the Airyscan, a so called ILEX (interleaved
laser excitation) mode is available.
This allows the tightest timing of the different
channels acquired with the Airyscan detector.
• To activate ILEX, use the ILEX Setup button
(Fig. 142) at the Airyscan multitrack section in
the Acquisition mode dialogue.
• You can either type in the required bandpass
emission filters or select them from the drop
down list. Then press the OK button (Fig. 143).
As a result, a linewise multitracking will be
configured automatically, which includes a 2x
linewise averaging of each image line acquired.
During this averaging, the excitation lasers will be
interleaved for best synchronicity.
• Make sure the correct laser lines are activated
by using the laser checkboxes for the respective
multitracks.
• The laser power can be set individually with the
slider (Fig. 144).
LSM 880
10/2014 V_01