Zeiss LSM 880 Operating Manual page 63

LSM 880
Visual inspection of the cells via binoculars
• In the main toolbar, activate the Locate tab.
• Choose from the Locate tab the Microscope Control tool.
For transmitted-light observation (Bright-field)
• Switch the HAL lamp on by the HAL On/Off button and adjust the light intensity.
• Activate the condenser function with the Condenser button if applicable.
• Press the Objective button to swing the required objective into the working position. We recommend
using the Plan-Apochromat 63x/1.4 oil for HR-SIM and 3D-PALM and the αPlan-Apochromat
100x/1.46 oil for PALM due to their optimized optics.
• Select the desired tube lens by clicking the Tube lens button. Normally, the correct Lens 1x will be
chosen automatically when the Locate tab of the main tool bar is pressed. When working with
Airyscan we also recommend using selected high NA objectives.
For reflected-light observation (Epi-fluorescence)
• Turn on the Excite power supply switch.
• Switch the Excite On/Off button to the On position. As needed switch the Excite Reflected Light
Open/Close Shutter into the Close/Open position.
• Select the desired filter cube in the reflector turret by clicking the Reflector button. The filter will be
automatically moved into the beam path to enable observation in epi-fluorescence.
• Select the desired tube lens by clicking the Tube lens button. Normally, the correct Lens 1x will be
chosen automatically when the Ocular tab is pressed.
• Swing the required objective into the working position by selecting the objective with the Objective
button. We recommend using the Plan-Apochromat 63x/1.4 oil for HR-SIM and 3D-PALM and the
αPlan-Apochromat 100x/1.46 oil for PALM due to their optimized optics. When working with Airyscan
we also recommend using selected high NA objectives.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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ZEISS
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