Recommendations For Excitation Laser Lines And Emission Filters Of Dyes - Zeiss LSM 880 Operating Manual

LSM 880

Recommendations for Excitation Laser Lines and Emission Filters of Dyes

4 Recommendations for Excitation Laser Lines and Emission Filters
of Dyes
Dye
DAPI
EBFP
Hoechst
Fluoro-Gold
ECFP
Lucifer Yellow
EGFP
FM 1-43™
Alexa Fluor 488™
Calcium Green
Cy2™
DiO (DiOC18(3))
Fluo-3
Fluorescein (FITC)
Cy3™
EYFP
Oregon Green
SYTOX Green
FM 4-46
Alexa Fluor 546™
Calcium Orange
DiI (DiIC18(3))
DsRed
Tetramethylrhodamine (TRITC)
Rhodamine B
Texas Red™
Alexa Fluor 633™
Cy5™
10/2014 V_01
CHAPTER 4 - ANNEX
Laser line/MBS
405
405
405
405 or 458
405 or 458
458
477 or 488
477 or 488
488
488
488
488
488
488
514/532
488 or 514/532
514/532
514/532
514 or 532/543
532/543
532/543
532/543
532/543 or 561
532/543 or 561
532/543
561 or 594
633/635
633/635
000000-2071-464
Emission bands
> 385/420, max. at 461
> 385/420, max. at 447
> 385/420, max. at 440
> 420/475, max. at 536
> 420/475, max. at 501
> 475, max. at 536
> 505, max. at 507/516
> 505, max. at 598
> 505, max. at 520
> 505, max. at 531
> 505, max. at 508
> 505, max. at 508
> 505, max. at 520
> 505, max. at 520
> 530, max. at 566
> 530, max. at 535
> 530, max. at 535
> 530, max. at 536
> 560, max. at 640
> 560, max. at 572
> 560, max. at 575
> 560, max. at 565
> 560, max. at 583
> 560, max. at 576
> 560/585, max. at 625
> 570/600, max. at 620
> 650, max. at 654
> 650, max. at 666
ZEISS
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