Z-Stack Settings For Airyscan - Zeiss LSM 880 Operating Manual

LSM 880
5.2.15.6

Z-Stack Settings for Airyscan

In case a 3-dimensional acquisition is required, a
Z-Stack (Fig. 200) can be defined the same way as
when using the internal channels.
However, in order to realize the resolution increase
of the SR mode in Z, a doubled sampling with
smaller Z intervals is required.
This is done automatically when the SR mode is
active.
Optimize Sectioning and Step, Optimal
In case the interval is defined manually, the
optimizing sectioning and step dialogue shows the
correct sectioning for the Z direction, and the
required oversampling for the active Airyscan
mode can be set by using the Optimal button
(Fig. 201).
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 200 Z-Stack tool: Airyscan settings
Fig. 201 Optimize sectioning and Step –
appearance for Airyscan mode
ZEISS
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