Ratio Imaging; Laser Control - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 107 Ratio imaging panel
Fig. 108 Color selection table
Fig. 109 Laser control panel
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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5.2.7.9

Ratio Imaging

In the Show all mode Ratio imaging becomes
available (only for WF and LSM imaging modes).
A click on the arrow next to Ratio provides access
to the Ratio channel interface panel (Fig. 107).
A maximum of two ratio channels (R1 and R2) can
be activated by ticking the respective check box.
These channels can display the ratio between two
of the active imaging channels.
The source channels for calculating the ratio have
to be selected from the Source 1 and Source 2
drop down menus. As an alternative to a second
channel for the ratio imaging it is also possible to
choose the 1st images of a time series of one
channel for the ratio metric calculation.
The parameters for the calculation are set in the
Channels tool of the Online Acquisition tool
group.
A suitable color can be assigned to each of the two
Ratio Channels R1 and R2 if their icons are pressed
(Fig. 108). The scroll bar allows access to all listed
colors.
The ratio to be formed between the selected
channels can be defined more precisely using
one of the four preset formulas in the
Channels tool of the Online Acquisition
tool group after a click on the relevant ratio
button (e.g.: R1). The Set by min/max
function (in Channels tool) allows the
definition of the display scaling according to
the expected minimal and maximal values.
5.2.7.10

Laser Control

Press the Laser button to display the laser control
expansion submenu (Fig. 109).
Activate the lasers you want to use by checking the
corresponding boxes.
The used laser line with sliders will appear.
Set the requested laser attenuation (%) using the
sliders, the arrows, or typing in the display field.
LSM 880
10/2014 V_01