Zeiss LSM 880 Operating Manual page 652

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Table of Contents

Introduction

OpticalImageFormation

Part 1 deals with the purely optical conditions

in a confocal LSM and the influence of the pin -

hole on image formation. From this, ideal

values for resolution and optical slice thickness

are derived.

PointSpreadFunction

ResolutionandConfocality

Geometric optic confocality

Wave-optical confocality

SignalProcessing

Part 2 limits the idealized view, looking at the

digitizing process and the noise introduced by

the light as well as by the optoelectronic

components of the system.

SamplingandDigitization

Types of A/D conversion

Nyquist theorem

Resolution and shot noise –

resolution probability

Possibilities to improve SNR

Part 3 deals with special aspects of Part 1

and 2 from a more physical perspective.

PupilIllumination

OpticalCoordinates

Fluorescence

SourcesofNoise

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