Zeiss LSM 880 Operating Manual page 218

ZEISS
D.3 Flow
Flow signifies active transport either via cytoplasmic movement or directed transport.
If flow occurs in the absence of translational diffusion, the term is defined as follows:
2
 
t
−    
t
t  
=
f
G ( ) e
f
In the presence of translational diffusion, the term alters to:
t
2
( )
t
− f
t
+
1 ( )
t t
=
G ( ) e
d
f
with t representing the average residence time for flow and t
f
Note, in the FCS software, the correct term is automatically loaded in dependence on the absence or
presence of a translational term.
With the knowledge of the lateral radius ω
the velocity v instead of the average residence time. The following relation exists:
ω
=
r
v
t
f
212
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
given as a fixed value, the software allows to fit directly to
r
000000-2071-464
the diffusion correlation time.
d
LSM 880
(8p)
(8q)
(8r).
10/2014 V_01