Zeiss LSM 880 Operating Manual page 537

LSM 880 Center Screen Area / Image Containers - Display and Image Analysis
In the Ratio1 channel choose the Ratio Type 3 formula. With Ch1-T1 and Ch1-T2 corresponding to I
and I this formula corresponds to the formula for anisotropy r. For the factors put in the value for the G-
s
factor in the numerator and 2 x G in the denominator. Scale by Min/Max by checking the Set by
min/max box and entering 0 in the Min input box and 1 in the Max input box. With a corresponding
look up table like rainbow this will produce a fasle colored anisotropy image in the Ratio1 channel.
Alternatively you can use the image calculator with source 1 (S1) being the P track image (Ch1-T1) and
source 2 (S2) the S track image (Ch1-T2) with consideration of the G-factor (see Fig. 750).
Proceed as follows. In the Processing tab select the Image calculator (Fig. 750). Press Select and click
on the desired input image. In the Channel pull down menu of Input Image Image 1 select ChX-T1,
where X stand for 1, 2 or S. Accordingly, in the Channel pull down menu of Input Image Image 2
select ChX-T2.
Type in the anisotropy formula considering the G-factor of the system. Choose either Absolute Intensities
in case you have to multiply the formula with 2
depth was 8 bit, multiply with 2
case you do not multiply with an extra factor. Press Apply and choose a respective look up table to
obtain a false colored anisotropy image.
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CHAPTER 1 - SYSTEM OPERATION
n
-1 to rescale to an n bit image. For example, if the color
8
-1=256-1=255. Alternatively, choose Intensity range 0..1, in which
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P
531