Zeiss LSM 880 Operating Manual page 309

LSM 880
For an overview of the available more advanced
operators, please refer to the formula panel that is
shown in a pop-up window after clicking the
Operators... button (Fig. 400).
By highlighting the selected operator, a description of
the function of this operator is displayed in the lower
part of the operators list (Fig. 400).
All image calculations in this tool work pixel by pixel and
start with the upper left pixel regardless of the image
size for the two images or image series.
When choosing images of different data depth,
the check box next to Intensity range 0...1
should be marked. This normalizes the image
intensity for all images to values between 0 and 1.
All actions performed in the Image Calculator have
immediate effect on the previews. After pressing the
Apply button on the top of the Processing main tab, a
new image document with the resulting image is
created.
A good example application for the Image Calculator is
offsetting/normalizing two images or an image series
with a reference image to enhance contrast for small
changes in intensity:
• Select a time series as Input 1 and the first frames of
these series (or another offset image) as Input 2.
• Then type/edit an operator as shown in Fig. 401 that
enhances the relative changes in the image with
respect to the relatively bright signal level, for
example:
S1-S2/2 = source 1 – ½ * source 2.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 400 Operators window
Fig. 401 Image calculation window
ZEISS
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