Processing - Image Calculator - Zeiss LSM 880 Operating Manual

ZEISS
5.4.5 Processing – Image Calculator
 standard
Tool accepts all types of image data recorded with the listed instruments. See full
description of tool for details.
Fig. 399 Processing – Image Calculator
The number in the First image display box, which can be edited, reduces the Input 2 Image series to
the selected first frames of a time series. This can be used to normalize a time series to the values of its
first image(s).
After loading 2 input images (source images S1 and S2) specifying the input channels, the calculator
keyboard in the Settings panel allows the definition of an operator or a combination of operators which
is represented by a formula in the formula display box. The operators will be inserted into the formula at
the cursor position. The formula can also be typed in directly. By pressing Input 1 or Input 2 source
image 1 or 2 will be loaded, respectively at the position of the cursor.
302
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
Licensing
optional
000000-2071-464
Instrument
 LSM  ELYRA
The Image calculator tool provides a calculator-
style interface to apply arithmetic operators to the
selected image.
• To open the Image calculator tool, click on the
Image calculator in the list of the Method
panel in the Processing main tool tab.
• Click on the triangle buttons to show or hide
the Method Parameters and Preview panels
(Fig. 399) for the function.
The two Select buttons in the Selection panel
select the active image in the Image Display as
Input Image and Input Image 2. The Output
Image is automatically fed into a new image
document. A specific input channel from a multi-
channel image or series can be selected using the
pull down menus next to the Select buttons.
 Lightsheet Z.1
10/2014 V_01
LSM 880