Zeiss LSM 880 Operating Manual page 140

ZEISS
Fig. 178 Shuttle and Find tool
Save marker images saves the marker images during the calibration. The images can be used to check
the calibration afterwards. Click on the Select Folder (...) button to select a storage folder.
• Configure the beam path manually or select an appropriate configuration from the list (Load
Acquisition Parameters).
• Click on the Continuous button to start the continuous scanning.
• Choose the options for marker recognition within the Marker Options field.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Calibration
The calibration markers consist of one small and a
large L-shape marker. The bigger marker is used
for coarse orientation, whereas the smaller marker
is used for the calibration.
• Set the microscope for eyepiece observation.
• Use a dry objective.
• Move the microscope stage to the marker
position 1 of the specimen holder.
• Focus on the small marker.
• Select the Acquire tab and open the Shuttle
and Find tool in the Acquisition Parameter
tool group (Fig. 178).
• Select the appropriate holder type from the list
by clicking the Select holder ... button, e.g.:
Life Science cover glass 22x22.
The currently selected holder type is then shown in
the Sample Holder: panel.
Apply to Image applies the current calibration to
an acquired image. Do not remove the sample of
the holder between image
calibration. Use this button only, when you forgot
to calibrate the holder before you acquired the
image.
• Choose general options for the calibration in
the Marker Options field:
Move stage in load position before x-y
movement moves the stage to load position
before moving to the next correlative calibration
marker. In case of using an Axio Observer, the
objective revolver moves to load position.
Automatic movement to next marker moves
the stage automatically to the next marker during
the calibration procedure if the position of the
correlative marker has been set manually or
confirmed (automatic marker detection). If this
checkbox is not activated, the stage has to be
moved manually to the next marker.
LSM 880
acquisition and
10/2014 V_01