Systemmaintenance Tool; Calibration Objective - Zeiss LSM 880 Operating Manual

LSM 880
2

SystemMaintenance Tool

With a calibration objective and correct system configuration, the SystemMaintenance tool allows
convenient self-adjustment of LSM.
The optical beampath, relative pinhole position and scanner adjustment can be set and checked
automatically with this tool. In systems that combine different imaging devices (e.g. ELYRA), the
SystemMaintenance tool calibrates only the LSM scan head.
2.1

Calibration Objective

Observe the following notes:
− The calibration objective is not suitable for multiphoton excitation; there is a risk of bubble formation
in the substrate.
− Using the HAL, the IR protection filter must be used, and the intensity of the HAL should only briefly
be set to more than 70 %.
− With strong heat there is a risk of deformation of the substrate in the calibration objective.
− The lateral screws need to be tightened carefully without applying too much force.
− The calibration objective needs to be entered in the system database as "APO Calibration LSM" with
SAP no. 420639-9000-700. It needs to be entered at ZEN \ Maintain \ Objectives and at the TFT
display.
− Calibration objectives with other samples than shown in Fig. 4 shall not be used. The cap with the
calibration sample can be obtained separately under SAP-No. 420639-9900-700.
10/2014 V_01
CHAPTER 3 - ADDITIONAL SOFTWARE TOOLS
SystemMaintenance Tool
Fig. 4 Calibration objective
000000-2071-464
ZEISS
3