Acquisition Parameter - Focus - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 168 Focus tool
The z-position can be altered by grapping and sliding the plane or by using the arrow slider. The plane
shows the actual z-position.
Pressing Manually in the Zero position field will set the current z-position to 0.
If a Z-Piezo stage insert is in use and active then pressing Leveling in the Piezo field will center the
Piezo stage position in regard to the microscope focus drive.
The Piezo Position (µm) input box allows the position of the Piezo to be altered.
The Step Size (µm) display box shows the currently used step size for focus movement of the Z-Piezo
stage.
Use of an optional high resolution focusing device (Piezo objective focus or Z-Piezo stage
insert)
The Piezo Position is indicated as a numerical value. This value and with it the focus position can be
changed. A change of this value updates the value of the microscope focus accordingly.
The Step Size, by which the Piezo focus moves when clicking on the arrows, can be set between
0.005 µm and 10 µm.
As soon as the focus position of the microscope is changed (via hand wheel or software), the piezo
objective focus is automatically levelled.
• A click on the Leveling button moves the Piezo objective focus or Z-Piezo stage insert into the center
position of its travel range and the focus position of the microscope is reset to compensate for the
leveling. Therefore, the same Z-level remains visible (the current position is not set to zero).
The motor focus of the stand is operated in the same way via the relevant buttons. Moving into the
Work or Load position is always performed via the motor focus and not via the Piezo objective focus.
Please see CHAPTER 4: ANNEX for further information on the Piezo objective focus and Z-Piezo
stage insert.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
5.2.10
If the Focus tool tab is expanded the Focus control
tool becomes available (Fig. 168).
The Z-Position display box shows the current
z-position.
The number can be edited by typing or using the
arrowheads. The nosepiece or stage will automatically
update the new position.
By pressing the Stop button, you can stop the current
movement. The Step Size (µm) display box shows
the currently used step size for focus movement. The
value can be edited by typing or using the
arrowheads.
The Home button sets the z-position to the
predefined zero value.
Pressing Load will lower the nose piece (z-position)
for objective change.
Work brings the z-position back to the actual used
position if the focus was zeroed or in Load position.
Home and Load positions can also be accessed from
the z-position display by pressing the H or L buttons.
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Acquisition Parameter – Focus
LSM 880
10/2014 V_01