Quantitative Colocalization Parameters Shown In The Data Table; Colocalization Coefficients - Zeiss LSM 880 Operating Manual

LSM 880 Center Screen Area / Image Containers - Display and Image Analysis
6.11.2

Quantitative Colocalization Parameters Shown in the Data Table

No. of pixels in image ROI or scatter region
−
Area / relative area of image ROI or scatter region
−
Mean intensities / SD within image ROI or scatter region
−

Colocalization coefficients

−
Weighted colocalization coefficients
−
Overlap coefficient after Manders
−
Correlation coefficients (R and R
−
6.11.3 Colocalization Coefficients
pixels
=
Ch , 1 coloc
c
1
pixels
Ch , 1 total
Relative number of colocalizing pixels in channel 1 or 2, respectively, as compared to the total
−
number of pixels above threshold.
Value range 0 – 1 (0: no colocalization, 1: all pixels colocalize)
−
All pixels above background count
−
Weighted colocalization coefficients
∑
Ch 1
i,coloc
=
i
M
∑
1
Ch 1
i,total
i
Sum of intensities of colocalizing pixels in channel 1 or 2, respectively, as compared to the overall
−
sum of pixel intensities above threshold and in this channel.
Value range 0 – 1 (0: no colocalization, 1: all pixels colocalize)
−
Bright pixels contribute more than faint pixels
−
Correlation coefficient, Pearson´s correlation coefficient
∑
−
( Ch 1 Ch
i
=
i
R
p
∑
−
( Ch 1 Ch 1
i
i
Provides information on the intensity distribution within the colocalizing region
−
Value range -1 to +1
−
-1,+1: all pixels are found on straight line in the scatter diagram
0: pixels in scattergram distribute in a cloud with no preferential direction
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
2
)
pixels
=
Ch , 2 coloc
c
2
pixels
Ch , 2
irrespective of their intensity.
∑
Ch 2
i,coloc
=
i
M
∑
2
Ch 2
i,total
i
−
1 ) ( * Ch 2 Ch 2
aver i aver
∑
−
2
) * ( Ch 2 Ch 2
aver i
i
000000-2071-464
total
)
2
)
aver
ZEISS
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