Zeiss LSM 880 Operating Manual page 101

LSM 880
Ratio Imaging Configuration
In the Show all mode of the Imaging Setup tool
two additional ratio imaging channels R1 and R2
can be defined. These channels are displayed in
addition to the maximum of 8 active imaging
channels. They provide the possibility to display the
ratio between two of the active channels.
A click on the arrow next to Ratio in the lower
part of the Imaging Setup tool window (Fig. 114)
provides access to the ratio channel interface. A
maximum of two ratio channels can be activated.
These channels can display the ratio between two of the active imaging channels. The source channels
for calculating the ratio have to be selected from the drop down menu next to Source 1 and Source 2. As
an alternative to a second channel for the ratio imaging is also possible to choose the first images of a
time series of one channel for the ratio metric calculation (e.g. to calculate F/F
dyes). The parameters for the calculation are set in the Channels tool (see section Acquisition
Parameter – Channels).
A suitable color is assigned to each of the two Ratio Channels R1 and R2, in the same way as for the
photomultiplier channels.
The ratio to be formed between the selected channels can be defined more precisely using one
of the four preset formulas in the Channels tool window after a click on the relevant ratio
button (e.g.: R1) for online display of radiometric or single wavelength dyes. The Set by min/max
function (in Channels tool window) allows the definition of the display scaling according to the
expected minimal and maximal values.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 114 Ratio channels in the
tool
ZEISS
Imaging Setup
for single wavelength
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