Zeiss LSM 880 Operating Manual page 245

LSM 880
If the XY Stage button is activated, all positioning will be done via the scanning stage.
This mode corresponds to the Sample Carrier mode, if Single Position is selected. Regardless
which well is selected, in the result table of the FCS results window the position is indicated
as "1".
(1) Current position
In Current Position you can select a position from a scanned image. The scanner will be automatically
taken for positioning.
• Click on the Current Position tab to open the Current Position panel (Fig. 318).
Cross Hair
When using this method, FCS measurements are
performed on the selected position. By triggering a
measurement the scanner will move to the
indicated position. If the Cross Hair was not
activated, the beam stays at the optical axis and if
no cropping or panning has been done this
position corresponds to the middle of the image. In
other cases the position of the laser beam with a
panning within the image has to be determined by
a bleach experiment. You can use the overlay tool
to indicate in the image where the laser position is.
Bleach a hole in a suitable sample like a dried dye
layer and record coordinates.
• Image your sample.
• Press the Cross Hair button (Fig. 319). This will
display a cross hair in your scan image. Click
again to remove the cross hair. Position the
crosshair at the coordinates you want to take
the reading. The crosshair will be positioned on
the site were you click with the left mouse
button. We recommend not to close the Scan
window after setting the crosshair. Then the
crosshair will be preserved within the new scan.
Fix the cross hair by pressing the Lock Cross
Hair button. This prevents the cross hair from
moving by an accidental click in the image
window.
Using Cross Hair the Scanner is used for
positioning.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 319 Current Position display with cross
hair activated (above) and image
with cross hair displayed (below)
ZEISS
239