Scan Overview Image For Tiling - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 209 Tile Scan: Marked positions
Fig. 210 Scan Overview Image window
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Marked positions are listed up. This list can be
saved and loaded.
5.2.18

Scan Overview Image for Tiling

Pressing the Scan overview Image... button
opens the Scan overview Image interactive window
(Fig. 210). Here you can define the number of
Tiles by using the Horizontal and Vertical Input
boxes (the extension of the scanned area will be
displayed in Pixel and Size (µm) units), the
objective from the Objective drop down menu
and a potential zoom factor via the Zoom slider or
Zoom input box with which to scan an overview
image. In the LSM imaging mode, a true zoom is
taken. For widefield modes, only the zoomed in
part of the camera chip will be displayed.
Changing Objective and Zoom will change the
size, but leaves Pixels unchanged. This overview
image can then be used to navigate in the sample
(i.e. define positions for imaging). Scan starts the
tile scan. Cancel closes the window without
further action.
LSM 880
10/2014 V_01