Zeiss LSM 880 Operating Manual page 65

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
Fig. 59 LSM 880 with Axio Observer.Z1
(3) Microscope settings on Axio Observer for reflected-light observation (Epi-fluorescence)
• Turn on the HBO 100 power supply switch (Fig. 59/1).
• Click on the Reflected Light button and set the shutter to Open.
• Click on the Reflector button and select the desired filter set by clicking on it.
• The filter is automatically moved into the beam path to enable observation in epi-fluorescence.
• Click on the Objective button and select the objective.
• Make sure that the illumination from the transmitted-light is switched off.
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