Zeiss LSM 880 Operating Manual page 250

ZEISS
Fig. 324 Position list with 3 positions
selected
Fig. 325 LSM scan image with 3 selected
positions
• Press the Remove button to remove selected (highlighted) positions from the Positions list and the
corresponding crossline in the scan image.
• Pres the Remove all button to remove all positions and all crosslines in the scan image.
• Press the Mark button to fix the crosslines as overlay elements in the scan image (Fig. 325). The
crosslines will also stay visible in the scan image if the Select button is deactivated. If you call up an
image later you have to activate the overlay function and a line drawing element to display the
crosslines.
Positioning is performed by scanning mirrors if the Scanner button or by the scanning stage, if the
XY Stage button is pressed. If more than one position was selected, measurement is only taken at
the highlighted position if the Snap action button is pressed. To approach all listed positions
sequentially the Start Experiment action button must be pressed.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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• Move the cursor to the desired position in the
scan image (drag and drop). Press the Add
button to add the position marked with the
crossline to the Positions list (Fig. 324). A
crossline is set at the selected cursor position in
the image (Fig. 325). To select further positions
click at the appropriate position in the image
and then press Add. If more than one position
is selected the crosslines are getting current
numbers. The Position list s displays the
numbers and coordinates of the positions in the
Number, x (µm), y (µm) and z (µm) display
boxes.
In a Z-Stack, you can also vary the Z-plane in
which measurements are to be performed. For
this purpose, select the required Z slice in the
stack or set the Z value via the focusing drive of
the microscope.
If the Scanner button is pressed, the scanner
will be positioned to the high-lighted
position if the Count Rate action button is
pressed. If the X,Y Stage button is pressed,
the stage will not be positioned to the high-
lighted position. Only after triggering a
measurement the stage will move to the
indicated position and will go back to the
initial position
measurement. If you want to obtain the
count rate by pressing the Count Rate
button at the indicated position using the
X,Y Stage control, you have to start a
measurement beforehand.
Positioning is only valid for the active (and
stored) scanned image. If different images
are open, be sure to
corresponding to your present sample.
LSM 880
after the end of
use the
10/2014 V_01
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