Zeiss LSM 880 Operating Manual page 635

LSM 880
Horizontal shift of the image generated with Beam1 (NLO laser) to the right
(To shift the image horizontally to the left, just use the reverse direction buttons):
1. Click on the right "fine movement" button of Beam1/Output/Axis2 repeatedly until an image
shift and illumination change is visible (image gets darker).
2. Now click on the right "fine movement" button of Beam1/Input/Axis2 repeatedly until the image
is bright again.
-> The image generated by the NLO laser now has shifted a small distance to the right.
3. Repeat steps 1 & 2 until the images of both beams are aligned horizontally.
If the offset is quite large, use the coarse buttons (not more than one click at once).
10/2014 V_01
CHAPTER 5 - MULTIPHOTON LASER SCANNING ...
NLO (Non Linear Optics) Laser for LSM
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ZEISS
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