Zeiss LSM 880 Operating Manual page 210

ZEISS
Blinking is based on the phenomenon that the electron distribution over conjugated systems can change
in dependence on the local environment, for example changes in the pH, which will lead to molecules in
a bright and dim or dark state. It is therefore a kinetic process that can be described in the following way
with the following relations:
←    →
k ; k
B F R D
1
t
=
b +
k k
F R
(
η η
⋅ ⋅ −
k k
= (
F R B
T
b
+ ⋅ ⋅ η
2
( k k ) k
F R F B
with B and D representing the brighter and darker states, k
rates and η and η
the emission yields or molecular brightness of molecule species (D or B) in Hz or the
B D
relative dimensionless brightness. In case, where darker state is completely dark (η
simplifies to
k
=
R
T
b
+
k k
F R
Note that Blinking is referred to a process that does not lead to a covalent modification in the chemical
bonds. If covalent changes occur the process is referred to as Flickering, which is formally treated in the
same way.
C.3 Dependent triplet and blinking
In this case the terms are just representatives for two dependent bunching terms that are linked by
addition (double exponential term). Note that the triplet fraction, if present, could be potentially fitted to
either of the terms.
t
−
t
= − + ⋅
G ( ) 1 ( T T e
t 1 1
t
−
⋅ +
t
T e
t 1
t
= +
1
G ( ) 1 (
t −
1 T
1
are the fractions of molecules in the triplet state, and t
where T and T
1 2
decay times.
, t and t
T , T are all fitted parameters.
1 2 t1 t2
204
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
)
2
)
D with the constraint
+ ⋅ η
2
k
R D
t
−
not normalized
− + ⋅
t t
T T e )
t 1 t 2
2 2
t
−
⋅
t
T e
t 2
normalized
2
)
−
T
2
000000-2071-464
η η
>
B D
and k the forward and backward reaction
F R
and t
t1
LSM 880
(7f)
(7g)
(7h)
=0), equation 7h
D
(7i)
(7j)
(7k)
the triplet exponential
t2
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