Zeiss LSM 880 Operating Manual page 102

ZEISS
Fig. 115 Imaging Setup tool window;
Lambda Mode activated
96
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
Activation / deactivation of the excitation wavelengths (check box) and setting of
excitation intensities (slider).
Selection of the main dichroic beam splitter (MBS) through selection from the
relevant list box.
Definition of number of PMTs out of the 34 PMT Detectors to fit the required
spectral range.
Access to the focus control (see section Focus Control)
Step width in spectral range (8.6 recommended).
Activation / deactivation of reflection.
000000-2071-464
Imaging in Lambda Mode
(to be selected in the Imaging Setup tool).
The Lambda Mode is recording the overall emission
from the sample onto a wavelength-dispersive
element and is imaged on a 34-channel detector. All
34 photomultipliers of the detector cover a spectral
width of approximately 360 nm, a single PMT covers a
spectral range of 8.9 nm. In the Lambda Mode,
images, image stacks, or time series are recorded in a
wavelength selective way.
This new image format is called Lambda Stack. For
the acquisition of a Lambda Stack, the complete
fluorescence signal can be read out at once. The
settings of the beam path for the Lambda Mode
scanning procedure with regard to the main dichroic
beam splitter and the QUASAR detector settings are
performed in the Imaging Setup tool (Fig. 115).
The QUASAR detector is displayed for spectral
imaging if Lambda Mode has been chosen as
imaging configuration.
The Imaging Setup tool displays the laser lines, the
main (dichroic) beam splitter, and the spectral range
of the QUASAR detector.
You can change the settings using the following
function elements:
LSM 880
10/2014 V_01